Overexpression of rice thaumatin-like protein (Ostlp) gene in transgenic cassava results in enhanced tolerance to Colletotrichum gloeosporioides f. sp. manihotis.
Identifieur interne : 000062 ( Main/Exploration ); précédent : 000061; suivant : 000063Overexpression of rice thaumatin-like protein (Ostlp) gene in transgenic cassava results in enhanced tolerance to Colletotrichum gloeosporioides f. sp. manihotis.
Auteurs : Patroba Odeny Ojola [Kenya] ; Evans N. Nyaboga [Kenya] ; Paul N. Njiru [Kenya] ; George Orinda [Kenya]Source :
- Journal, genetic engineering & biotechnology [ 2090-5920 ] ; 2018.
Abstract
Cassava (Manihot esculenta Crantz) is the most important staple food for more than 300 million people in Africa, and anthracnose disease caused by Colletotrichum gloeosporioides f. sp. manihotis is the most destructive fungal disease affecting cassava production in sub-Saharan Africa. The main objective of this study was to improve anthracnose resistance in cassava through genetic engineering. Transgenic cassava plants harbouring rice thaumatin-like protein (Ostlp) gene, driven by the constitutive CaMV35S promoter, were generated using Agrobacterium-mediated transformation of friable embryogenic calli (FEC) of cultivar TMS 60444. Molecular analysis confirmed the presence, integration, copy number of the transgene all the independent transgenic events. Semi-quantitative RT-PCR confirmed high expression levels of Ostlp in six transgenic lines tested. The antifungal activity of the transgene against Colletotrichum gloeosporioides pathogen was evaluated using the leaves and stem cuttings bioassay. The results demonstrated significantly delayed disease development and reduced size of necrotic lesions in leaves and stem cuttings of all transgenic lines compared to the leaves and stem cuttingss of non-transgenic control plants. Therefore, constitutive overexpression of rice thaumatin-like protein in transgenic cassava confers enhanced tolerance to the fungal pathogen C. gloeosporioides f. sp. manihotis. These results can therefore serve as an initial step towards genetic engineering of farmer-preffered cassava cultivars for resistance to anthracnose disease.
DOI: 10.1016/j.jgeb.2017.12.002
PubMed: 30647714
PubMed Central: PMC6296589
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Overexpression of rice thaumatin-like protein (<i>Ostlp</i>) gene in transgenic cassava results in enhanced tolerance to <i>Colletotrichum gloeosporioides</i> f. sp. <i>manihotis</i>.</title><author><name sortKey="Odeny Ojola, Patroba" sort="Odeny Ojola, Patroba" uniqKey="Odeny Ojola P" first="Patroba" last="Odeny Ojola">Patroba Odeny Ojola</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Nyaboga, Evans N" sort="Nyaboga, Evans N" uniqKey="Nyaboga E" first="Evans N" last="Nyaboga">Evans N. Nyaboga</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Njiru, Paul N" sort="Njiru, Paul N" uniqKey="Njiru P" first="Paul N" last="Njiru">Paul N. Njiru</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture and Resource Management, University of Embu, P.O. Box 6 - 60100, Embu, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Agriculture and Resource Management, University of Embu, P.O. Box 6 - 60100, Embu</wicri:regionArea><wicri:noRegion>Embu</wicri:noRegion></affiliation></author><author><name sortKey="Orinda, George" sort="Orinda, George" uniqKey="Orinda G" first="George" last="Orinda">George Orinda</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30647714</idno><idno type="pmid">30647714</idno><idno type="doi">10.1016/j.jgeb.2017.12.002</idno><idno type="pmc">PMC6296589</idno><idno type="wicri:Area/Main/Corpus">000046</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000046</idno><idno type="wicri:Area/Main/Curation">000046</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000046</idno><idno type="wicri:Area/Main/Exploration">000046</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of rice thaumatin-like protein (<i>Ostlp</i>) gene in transgenic cassava results in enhanced tolerance to <i>Colletotrichum gloeosporioides</i> f. sp. <i>manihotis</i>.</title><author><name sortKey="Odeny Ojola, Patroba" sort="Odeny Ojola, Patroba" uniqKey="Odeny Ojola P" first="Patroba" last="Odeny Ojola">Patroba Odeny Ojola</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Nyaboga, Evans N" sort="Nyaboga, Evans N" uniqKey="Nyaboga E" first="Evans N" last="Nyaboga">Evans N. Nyaboga</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author><author><name sortKey="Njiru, Paul N" sort="Njiru, Paul N" uniqKey="Njiru P" first="Paul N" last="Njiru">Paul N. Njiru</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture and Resource Management, University of Embu, P.O. Box 6 - 60100, Embu, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Agriculture and Resource Management, University of Embu, P.O. Box 6 - 60100, Embu</wicri:regionArea><wicri:noRegion>Embu</wicri:noRegion></affiliation></author><author><name sortKey="Orinda, George" sort="Orinda, George" uniqKey="Orinda G" first="George" last="Orinda">George Orinda</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya.</nlm:affiliation><country xml:lang="fr">Kenya</country><wicri:regionArea>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi</wicri:regionArea><wicri:noRegion>Nairobi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal, genetic engineering & biotechnology</title><idno type="eISSN">2090-5920</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cassava (<i>Manihot esculenta</i> Crantz) is the most important staple food for more than 300 million people in Africa, and anthracnose disease caused by <i>Colletotrichum gloeosporioides</i> f. sp. <i>manihotis</i> is the most destructive fungal disease affecting cassava production in sub-Saharan Africa. The main objective of this study was to improve anthracnose resistance in cassava through genetic engineering. Transgenic cassava plants harbouring rice thaumatin-like protein (<i>Ostlp</i>) gene, driven by the constitutive CaMV35S promoter, were generated using <i>Agrobacterium</i>-mediated transformation of friable embryogenic calli (FEC) of cultivar TMS 60444. Molecular analysis confirmed the presence, integration, copy number of the transgene all the independent transgenic events. Semi-quantitative RT-PCR confirmed high expression levels of <i>Ostlp</i> in six transgenic lines tested. The antifungal activity of the transgene against <i>Colletotrichum gloeosporioides</i> pathogen was evaluated using the leaves and stem cuttings bioassay. The results demonstrated significantly delayed disease development and reduced size of necrotic lesions in leaves and stem cuttings of all transgenic lines compared to the leaves and stem cuttingss of non-transgenic control plants. Therefore, constitutive overexpression of rice thaumatin-like protein in transgenic cassava confers enhanced tolerance to the fungal pathogen <i>C. gloeosporioides</i> f. sp. <i>manihotis</i>. These results can therefore serve as an initial step towards genetic engineering of farmer-preffered cassava cultivars for resistance to anthracnose disease.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30647714</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2090-5920</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal, genetic engineering & biotechnology</Title><ISOAbbreviation>J Genet Eng Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Overexpression of rice thaumatin-like protein (<i>Ostlp</i>) gene in transgenic cassava results in enhanced tolerance to <i>Colletotrichum gloeosporioides</i> f. sp. <i>manihotis</i>.</ArticleTitle><Pagination><MedlinePgn>125-131</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jgeb.2017.12.002</ELocationID><Abstract><AbstractText>Cassava (<i>Manihot esculenta</i> Crantz) is the most important staple food for more than 300 million people in Africa, and anthracnose disease caused by <i>Colletotrichum gloeosporioides</i> f. sp. <i>manihotis</i> is the most destructive fungal disease affecting cassava production in sub-Saharan Africa. The main objective of this study was to improve anthracnose resistance in cassava through genetic engineering. Transgenic cassava plants harbouring rice thaumatin-like protein (<i>Ostlp</i>) gene, driven by the constitutive CaMV35S promoter, were generated using <i>Agrobacterium</i>-mediated transformation of friable embryogenic calli (FEC) of cultivar TMS 60444. Molecular analysis confirmed the presence, integration, copy number of the transgene all the independent transgenic events. Semi-quantitative RT-PCR confirmed high expression levels of <i>Ostlp</i> in six transgenic lines tested. The antifungal activity of the transgene against <i>Colletotrichum gloeosporioides</i> pathogen was evaluated using the leaves and stem cuttings bioassay. The results demonstrated significantly delayed disease development and reduced size of necrotic lesions in leaves and stem cuttings of all transgenic lines compared to the leaves and stem cuttingss of non-transgenic control plants. Therefore, constitutive overexpression of rice thaumatin-like protein in transgenic cassava confers enhanced tolerance to the fungal pathogen <i>C. gloeosporioides</i> f. sp. <i>manihotis</i>. These results can therefore serve as an initial step towards genetic engineering of farmer-preffered cassava cultivars for resistance to anthracnose disease.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Odeny Ojola</LastName><ForeName>Patroba</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nyaboga</LastName><ForeName>Evans N</ForeName><Initials>EN</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Njiru</LastName><ForeName>Paul N</ForeName><Initials>PN</Initials><AffiliationInfo><Affiliation>Department of Agriculture and Resource Management, University of Embu, P.O. Box 6 - 60100, Embu, Kenya.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Orinda</LastName><ForeName>George</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>01</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Genet Eng Biotechnol</MedlineTA><NlmUniqueID>101317150</NlmUniqueID><ISSNLinking>1687-157X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Colletotrichum gloeosporioides f. sp. manihotis</Keyword><Keyword MajorTopicYN="N">Thaumatin-like protein</Keyword><Keyword MajorTopicYN="N">Transgenic cassava</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>08</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>12</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>1</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>1</Month><Day>17</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30647714</ArticleId><ArticleId IdType="doi">10.1016/j.jgeb.2017.12.002</ArticleId><ArticleId IdType="pii">S1687-157X(17)30116-6</ArticleId><ArticleId IdType="pmc">PMC6296589</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Trends Plant Sci. 1999 Jun;4(6):226-231</Citation><ArticleIdList><ArticleId IdType="pubmed">10366879</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2001 Aug;4(4):309-14</Citation><ArticleIdList><ArticleId IdType="pubmed">11418340</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycopathologia. 2002;154(4):191-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12206320</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2002 Oct;15(10):1031-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12437301</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2003 Mar;54(384):1101-11</Citation><ArticleIdList><ArticleId IdType="pubmed">12598580</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1992 Jul;2(4):465-75</Citation><ArticleIdList><ArticleId IdType="pubmed">1344886</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2004 Nov;56(4):503-16</Citation><ArticleIdList><ArticleId IdType="pubmed">15630615</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2007 May;26(5):631-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17103003</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2007 Aug;26(8):1309-20</Citation><ArticleIdList><ArticleId IdType="pubmed">17377794</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2010 May;29(5):419-36</Citation><ArticleIdList><ArticleId IdType="pubmed">20204373</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2011;62:251-72</Citation><ArticleIdList><ArticleId IdType="pubmed">21526968</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Biochem Biotechnol. 2012 Feb;166(4):1008-19</Citation><ArticleIdList><ArticleId IdType="pubmed">22183565</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Dec 24;4:526</Citation><ArticleIdList><ArticleId IdType="pubmed">24400011</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2015 Jun 10;6:411</Citation><ArticleIdList><ArticleId IdType="pubmed">26113851</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1994 May 1;218(2):474-6</Citation><ArticleIdList><ArticleId IdType="pubmed">7521147</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Kenya</li></country></list><tree><country name="Kenya"><noRegion><name sortKey="Odeny Ojola, Patroba" sort="Odeny Ojola, Patroba" uniqKey="Odeny Ojola P" first="Patroba" last="Odeny Ojola">Patroba Odeny Ojola</name></noRegion><name sortKey="Njiru, Paul N" sort="Njiru, Paul N" uniqKey="Njiru P" first="Paul N" last="Njiru">Paul N. Njiru</name><name sortKey="Nyaboga, Evans N" sort="Nyaboga, Evans N" uniqKey="Nyaboga E" first="Evans N" last="Nyaboga">Evans N. Nyaboga</name><name sortKey="Orinda, George" sort="Orinda, George" uniqKey="Orinda G" first="George" last="Orinda">George Orinda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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